The Litesizer 500 is a measuring instrument in which dynamic light scattering (DLS), electrophoretic light scattering (ELS), and static light scattering (SLS) are combined in a compact structure. This combination enables the Litesizer 500 to determine a range of important paramaters for particles, droplets and macromolecules dispersed in liquid, including:
- particle size (hydrodynamic diameter and size distribution)
- particle charge and the chemical surface functionalities of the particles via the zeta potential and zeta potential distribution
- molecular masses of polymers, proteins and nanoparticles
- particle interactions from the second virial coefficient
- turbidity of the sample (transmittance at 658 nm)
- refractive index of the continuous medium (using known references)
Technical specifications
| |
Litesizer 500 |
| Particle size specifications |
| Measurement principle |
Dynamic Light Scattering (DLS) |
| Measuring range |
0.3 nm to 10 µm (particle diameter) |
| Measurement angles |
15°, 90°, 175° |
| Min. concentration |
0.1 mg/mL (lysozyme) |
| Max. concentration |
50 %w/v (sample-dependent) |
| Min. sample volume |
12 µL |
| Accuracy |
Better than +/-2 % on NIST traceable standards |
| Repeatability |
Better than +/-2 % on NIST traceable standards |
| Zeta potential specifications |
| Measuring principle |
Electrophoretic Light Scattering (ELS) / cmPALS |
| Measuring range |
>= +/-1000 mV |
| Mobility range |
10 -11 m²/V.s to 2 x 10 -7 m²/V.s |
| Size range |
3.8 nm to 100 µm (diameter) |
| Min. sample concentration |
0.1 mg/mL (lysozyme) |
| Max. sample concentration |
70 %w/v (sample-dependent) |
| Max. sample conductivity |
200 mS/cm |
| Min. sample volume |
50 µL (sample-viscosity-dependent) |
| Accuracy |
Better than +/-10 % |
| Repeatability |
+/-3 % |
| Molecular mass specifications |
| Measuring principle |
Static Light Scattering (SLS) |
| Measuring range (mass) |
980 Da to 20 MDa |
| Measuring range (particle size) |
up to 40 nm (diameter) |
| Measurement angle |
90° |
| Min. sample concentration |
0.1 mg/mL (lysozyme) |
| Accuracy |
+/-10 % |
| Repeatability |
+/-5 % |
| Transmittance specifications |
| Measuring range |
0 % to 100 % |
| Min. sample volume |
15 µL |
| Accuracy |
Better than +/-1 % |
| Refractive index specifications |
| Measuring range |
1.28 to 1.50 |
| Min. sample volume |
1 mL |
| Accuracy |
+/-0.5 % |
| General specifications |
| Light source |
Semiconductor laser diode / 40 mW, 658 nm |
| Laser warm-up time |
6 min |
| Temperature control range |
0 °C to 90 °C |
| Ambient operating temperature |
10 °C to 35 °C |
| Humidity |
up to 80 % non-condensing |
| Dimensions (W, D, H) |
460 mm, 485 mm, 135 mm |
| Weight |
approx. 18 kg (40 lb) |